Introduction

ADONIX is the respiratory part of the population based INTERGENE research program assessing the INTERplay between GENEtic susceptibility and external factors such as environmental conditions, lifestyle, and biological and psychosocial background for the risk of chronic disease. The INTERGENE study includes 3614 individuals, aged 25 to 75 years, living in the south-western part of Sweden. The ADONIX cohort comprises of 6685 individuals of whom 2478 is also included in the INTERGENE cohort. The primary aim of the ADONIX study is to examine a biomarker for airway inflammation (FENO), with specific attention to asthma and chronic obstructive pulmonary disease (COPD), and interactions with genetic susceptibility. Baseline examinations were conducted between 2001 and 2008, including questionnaires, spirometry and FENO. During 2005-2006 a follow-up questionnaire was sent out including questions on respiratory and allergic disease, on environmental factors and on employment status. A re-examination was done between 2014 and 2016 comprising of the full study protocol conducted at the baseline visit, together with measurements of endogenous particles in exhaled air assessed by the PExA (Particles in Exhaled Air) method. At both visits ambient air pollution data were collected from monitoring stations in Gothenburg and the county and linked to each participants examination day and place of residence.

In 2023, a new sub-project was initiated based on the hypothesis that the coagulation system in the small airway lining fluid is affected following exposure to traffic-related emissions. Moreover, these effects are amplified in extra vulnerable individuals, such as individuals with COPD, and can result in secondary effects in other organ systems. This study is currently ongoing at the Department of Occupational and Environmental Medicine in Gothenburg. The goal is to recruit 40 individuals with COPD and 40 healthy controls from previous ADONIX participants. A comprehensive clinical examination of lung function and collection of a sample from the respiratory tract lining fluid with PExA are performed, in addition to home monitoring of particle matter exposure for three days before participation.  

Investigators

• Anna-Carin Olin, current principal investigator, Occupational -and Environmental medicine, Gothenburg University
• Kjell Torén, former principal investigator, Occupational -and Environmental medicine, Gothenburg University

Responsible university or institution

Occupational and Environmental Medicine, School of Public Health and Community Medicine, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg

Collaborators

• INTERGENE
• PURE (Prospective Urban and Rural Epidemiological Study)

Funders

• Swedish Heart and Lung foundation
• The Swedish Research Council Formas

How to collaborate with ADONIX

We are happy to welcome potential collaborators! Please contact Professor Anna-Carin Olin, principal investigator in the ADONIX study, for further information.

Contact information

Anna-Carin Olin
anna-carin.olin@amm.gu.se
+46 70 655 49 40

Key publications

Bouma F, Nyberg F, Olin A-C, Krage Carlsen H. Genetic susceptibility to airway inflammation and exposure to short-term outdoor air pollution. Environ Health. 2023 Jun 29;22(1):50. doi:

Brisman J, Kim JM, Olin A-C, Bake B, Toren K. Spirometric reference equations for Swedish adults. Clin phys funct Imag, 2017. 37; 6: 640-645 10.1186/s12940-023-00996-7.

Brisman, J. Kim JL, Olin AC, Torén K, Bake B. A physiologically based model for spirometric reference equations in adults. Clin Physiol Funct Imaging, 2016. 36(1): 77-84.

Mehlig K, Berg C, Björck L, Nyberg F, Olin AC, Rosengren A, Strandhagen E, Torén K, Thelle DS, Lissner L. Cohort Profile: The INTERGENE Study. Int J Epidemiol. 2017 Dec 1;46(6):1742-1743h. doi: 10.1093/ije/dyw332.PMID: 28186561 

Carlsen HK, Modig L, Levinsson A, Kim JL, Toren K, Nyberg F, Olin AC. Exposure to traffic and lung function in adults: a general population cohort study. BMJ Open. 2015 Jun 24;5(6):e007624. doi: 10.1136/bmjopen-2015-007624.PMID: 26109116 Free PMC article.

Carlsen, HK, Nyberg, F, Toren, K, Segersson, D, Olin, AC. Exposure to traffic-related particle matter and effects on lung function and potential interactions in a cross-sectional analysis of a cohort study in west Sweden. BMJ Open 2020; 10:10.

Dahgam S, Nyberg F, Modig L, Naluai AT, Olin AC. Single nucleotide polymorphisms in the NOS2 and NOS3 genes are associated with exhaled nitric oxide. J Med Genet. 2012;49:200-5.

Berg CM, Thelle DS, Rosengren A, Lissner L, Torén K, Olin AC. Decreased fraction of exhaled nitric oxide in obese subjects with asthma symptoms: data from the population study INTERGENE/ADONIX. Chest. 2011 May;139(5):1109-1116. doi: 10.1378/chest.10-1299. Epub 2010 Dec 23.PMID: 21183611

Levinsson A, Olin A-C, Björck L, Rosengren A, Nyberg F. Nitric oxide synthase (NOS) single nucleotide polymorphisms are associated with coronary heart disease and hypertension in the INTERGENE study. Nitric Oxide 2014;39: 1-7

Levinsson A, Olin A-C, Björck L, Rosengren A, Nyberg F. Interaction Effects of Long-Term Air Pollution Exposure and Variants in the GSTP1, GSTT1 and GSTCD Genes on Acute Myocardial Infarction and Hypertension: a Case-Control Study. PLOS ONE 2014;9(6): 8.

Modig L, Dahgam S, Olsson D, Nyberg F, Forsberg B, Olin A-C. Short-Term Exposure to Ozone is Associated with Increased Alveolar Nitric Oxide. Epidemiology 2013; 25:79-87.

Olin AC, Rosengren A, Thelle DS, Lissner L, Torén K. Increased fraction of exhaled nitric oxide predicts new-onset wheeze in a general population. Am J Respir Crit Care Med. 2010 Feb 15;181(4):324-7. doi: 10.1164/rccm.200907-1079OC. Epub 2009 Dec 10.PMID: 20007926

Olin AC, Bake B, Torén K. Fraction of exhaled nitric oxide at 50 mL/s: reference values for adult lifelong never-smokers. Chest 2008; 133: 831-832

Olin AC, Rosengren A, Thelle DS, Lissner L, Bake B, Torén K. Height, age, and atopy are associated with fraction of exhaled nitric oxide in a large adult general population sample. Chest. 2006 Nov;130(5):1319-25. doi: 10.1378/chest.130.5.1319.PMID: 17099006

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